A Validated HPTLC Method for Estimation of Moxifloxacin Hydrochloride in Tablets

Abstract

A simple HPTLC method having high accuracy, precision and reproducibility was developed for the routine estimation of moxifloxacin hydrochloride  tablets validated according to International Conference on Harmonization (ICH) guidelines. Moxifloxacin hydrochloride was estimated at 292 nm by densitometry using Silica gel 60 F₂₅₄ as stationary phase and a premix of methylene chloride: methanol: strong ammonia solution and acetonitrile (10:10:5:10) as mobile phase. The method was found linear in a range of 9 nanograms to 54 nanograms. The correlation coefficient in between the responses observed and respective drug amounts applied was found above 0.99. The regression equation generated was- Area under the curve = 65.57 ´ (Amount applied in nanograms) + 163.